Some Limits to Global Ecophagy
by Biovorous Nanoreplicators,
with Public Policy Recommendations

The maximum rate of global ecophagy by biovorous self-replicating nanorobots is fundamentally restricted by the replicative strategy employed; by the maximum dispersal velocity of mobile replicators; by operational energy and chemical element requirements; by the homeostatic resistance of biological ecologies to ecophagy; by ecophagic thermal pollution limits (ETPL);  and most importantly by our determination and readiness to stop them. Assuming current and foreseeable energy-dissipative designs requiring ~100 MJ/kg for chemical transformations (most likely for biovorous systems), ecophagy that proceeds slowly enough to add ~4°C to global warming (near the current threshold for immediate climatological detection) will require ~20 months to run to completion; faster ecophagic devices run hotter, allowing quicker detection by policing authorities. All ecophagic scenarios examined appear to permit early detection by vigilant monitoring, thus enabling rapid deployment of effective defensive instrumentalities.

1.0 Introduction

Perhaps the earliest-recognized and best-known danger of molecular nanotechnology is the risk that self-replicating nanorobots capable of functioning autonomously in the natural environment could quickly convert that natural environment (e.g., "biomass") into replicas of themselves (e.g., "nanomass") on a global basis, a scenario usually referred to as the "gray goo problem" but perhaps more properly termed "global ecophagy." [Also Also]

As Drexler first warned in Engines of Creation [2]:

"Plants" with "leaves" no more efficient than today's solar cells could out-compete real plants, crowding the biosphere with an inedible foliage. Tough omnivorous "bacteria" could out-compete real bacteria: They could spread like blowing pollen, replicate swiftly, and reduce the biosphere to dust in a matter of days. Dangerous replicators could easily be too tough, small, and rapidly spreading to stop - at least if we make no preparation. We have trouble enough controlling viruses and fruit flies.

Among the cognoscenti of nanotechnology, this threat has become known as the "gray goo problem." Though masses of uncontrolled replicators need not be gray or gooey, the term "gray goo" emphasizes that replicators able to obliterate life might be less inspiring than a single species of crabgrass. They might be superior in an evolutionary sense, but this need not make them valuable.

The gray goo threat makes one thing perfectly clear: We cannot afford certain kinds of accidents with replicating assemblers.

Gray goo would surely be a depressing ending to our human adventure on Earth, far worse than mere fire or ice, and one that could stem from a simple laboratory accident.

Lederberg [3] notes that the microbial world is evolving at a fast pace, and suggests that our survival may depend upon embracing a "more microbial point of view." The emergence of new infectious agents such as HIV and Ebola demonstrates that we have as yet little knowledge of how natural or technological disruptions to the environment might trigger mutations in known organisms or unknown extant organisms [81], producing a limited form of "green goo" [92].

However, biovorous nanorobots capable of comprehensive ecophagy will not be easy to build and their design will require exquisite attention to numerous complex specifications and operational challenges.  Such biovores can emerge only after a lengthy period of purposeful focused effort, or as a result of deliberate experiments aimed at creating general-purpose artificial life, perhaps by employing genetic algorithms, and are highly unlikely to arise solely by accident.
 

2.0 The Ecophagic Threat

However, atoms of Al, Ti and B are far more abundant in the Earth's crust (81,300 ppm, 4400 ppm and 3 ppm, respectively [5]) than in biomass, e.g., the human body (0.1 ppm, 0 ppm, and 0.03 ppm [6]), reducing the direct threat of ecophagy by such systems (Section 8.3). On the other hand, carbon is a thousand times less abundant in crustal rocks (320 ppm, mostly carbonates) than in the biosphere (~230,000 ppm).

Furthermore, conversion of the lithosphere into nanomachinery is not a primary concern because ordinary rocks typically contain relatively scarce sources of energy. For instance, natural radioactive isotopes present in crustal rocks vary greatly as a function of the geological composition and history of a region, but generally range from 0.15-1.40 mGy/yr [7], giving a raw power density of 0.28-2.6 ×10^-7 W/m³ assuming crustal rocks of approximately mean terrestrial density (5522 kg/m³ [5]). This is quite insufficient to power nanorobots capable of significant activities; current nanomachine designs typically require power densities on the order of 10⁵-10⁹ W/m³ to achieve effective results [6]. (Biological systems typically operate at 10²-10⁶ W/m³ [6].) Solar power is not readily available below the surface, and the mean geothermal heat flow is only 0.05 W/m² at the surface [6], just a tiny fraction of solar insolation. Subsurface pressure and temperature rise with depth in Earth's crust at the rates of 0.47 atm/meter and k_q ~ 0.014°K/meter [8], exceeding maximum reasonable nanorobot operating limits of 100,000 atm and 2000°K at depths of ~210 km and ~120 km well into the upper mantle below a ~50 km crust; however, geothermal power density is only D_p ~ K_t k_q²k_Carnot / DT ~ 1-4 ×10^-6 W/m³ taking thermal conductivity K_t ~ 2-5 W/m-K for common crustal minerals [9] and DT ~ 1°C giving Carnot efficiency k_Carnot = DT / T ~ 0.3% at T = 300°K.

Hypothesized crustal abiotic highly-reduced petroleum reserves [16] probably could not energize significant replicator nanomass growth due to the anoxic environment deep underground, although potentially large geobacterial populations have been described [10-16] and in principle some unusual though highly limited bacterial energy sources could also be tapped by nanorobots. For example, some anaerobic bacteria use metals (instead of oxygen) as electron-acceptors [13], with iron present in minerals such as pyroxene or olivine being converted to iron in a more oxidized state in magnetic minerals such as magnetite and maghemite, and using geochemically produced hydrogen to reduce CO₂ to methane [11]. Underground bacteria in the Antrim Shale deposit produce 1.2 ×10⁷ m³/day of natural gas (methane) by consuming the 370 MY-old remains of ancient algae [17]. Bioremediation experiments have also been done by Envirogen and others in which pollution-eating bacteria are purposely injected into the ground to metabolize organic toxins; in field tests it has proven difficult to get the bacteria to move through underground aquifers, because the negatively-charged cells tend to adhere to positively charged iron oxides in the soil [18].

However, the primary ecophagic concern is that runaway nanorobotic replicators or "replibots" will convert the entire surface biosphere (the ecology of all living things on the surface of the Earth) into alternative or artificial materials of some type -- especially, materials like themselves, e.g., more self-replicating nanorobots. Since advanced nanorobots might be constructed predominantly of carbon-rich diamondoid materials [4], and since ~12% of all atoms in the human body (representative of biology generally) are carbon atoms [6], or ~23% by weight, the global biological carbon inventory may support the self-manufacture of a final mass of replicating diamondoid nanorobots on the order of ~0.23 M_bio, where M_bio is the total global biomass.

Unlike almost any other natural material, biomass can serve both as a source of carbon and as a source of power for nanomachine replication. Ecophagic nanorobots would regard living things as environmental carbon accumulators, and biomass as a valuable ore to be mined for carbon and energy. Of course, biosystems from which all carbon has been extracted can no longer be alive but would instead become lifeless chemical sludge.
 

3.0 Exponential Replication Rate

for maximum exponential growth, where t is elapsed time (sec), t is generation cycle or replication time (sec), M_init (kg) is initial nanorobot mass at time t = 0, and M_repl (kg) is the replicator mass at time t, where M_repl 0.23 M_bio.    In order to achieve this rate, each completed component of the unit currently being built must be put to full productive use immediately, instead of waiting for the final completion of the unit.  There are a few design configurations where something close to this can be achieved efficiently, but as a practical matter and to retain simplicity it will usually be preferable to await the completion of a unit before pressing it into replicative service, a mode of operation called discrete replication, in which case the exponential term in Eqn. 1 should be replaced with 2^(t/tdiscrete⁾ -- which, all else equal, will be a slightly slower function.  (Discrete replication can be faster than pure exponential replication only if t_discrete < t ln(2).)  Replicating populations limited to activity only at the perimeter of the expansion wave, or in regions of high replibot number density, may achieve only polynomial growth rates [19], which are even slower.

In order to estimate t = t_conv, the time required for total conversion of the biosphere to replibots plus waste sludge, we must first estimate t. Drexler [4] has calculated that a readily-envisioned multistage molecular manufacturing system could manufacture its own mass in t ~ 1000 seconds. However, nanoreplicators need not be capable of general purpose manufacturing, but may be optimized solely for replication of their own substance. A molecular manipulator designed by Drexler [4] that is suitable for molecular assembly pick-and-place operations consists of 4 million atoms excluding support base, power, control, and other necessary structures, and is designed to perform ~10⁶ atomic-precision molecular pick-and-place operations per second, assuming arm-tip movement at 1 cm/sec over minimal 10-nm arcs each cycle. Freitas [6] estimates that a basic autonomous nanoassembler using two Drexler manipulator arms and incorporating a simple onboard nanocomputer might require at least ~70 million atoms (~1 gigadalton), suggesting a minimum replication time t ~ 100 seconds. (The smallest independently viable cells are thought to have a molecular weight of order ~1 gigadalton, e.g., minimum diameter ~140 nm[72, 73].)

It is difficult to imagine how an ecophagic replicator capable of successfully assimilating natural biomatter of all existing varieties could be much simpler than this. However, it is possible that molecular manipulators might be slewed at speeds up to ~100 cm/sec, perhaps giving t ~ 1 sec, but at the cost of steeply rising energy dissipation [4] which greatly increases waste heat production and system operating temperatures, and reduces nanoreplicator reliability due to larger thermally-excited displacements, thermal damage rates, and phonon-mediated drag [4]. For example, a 10-nm force sensor measuring 10 pN at an operating temperature of 300°K has a 0.2% probability of erroneous measurement; this probability jumps to 3% at 500°K and 16% at 1000°K [4]. Hence, t ~ 1 sec appears to be a rather aggressive and probably unachievable lower limit.
 

Carbon inventory in the global biomass has been estimated as 1.1 ×10¹⁵ kg (Table 1). Life is ~23% carbon by weight, so the total global biomass can be estimated as M_bio ~ 5 ×10¹⁵ kg. Starting from a single 70-million-atom replicator of mass ~1 gigadalton [6] or M_init ~ 1.7 ×10^-18 kg, and taking t ~ 1 sec and M_repl = 0.23 M_bio ~ 1.2 ×10¹⁵ kg, then t_conv ~ 76 sec. Adopting a more reasonable t ~ 100 sec, t_conv ~ 7600 sec. For comparison, the fastest known replicators found in nature are certain bacteria which have a mean generation time of t ~ 900-1200 sec (15-20 minutes) [27]. However, these bacteria are capable of digesting only certain limited forms of biological matter and have very severe operational restrictions including proper temperature, pH, and so forth. They are not bio-omnivorous.
 

4.0 Dispersal Velocity Limitation

assuming t_spread >> t, where the mean planetary radius R_Earth = 6.37 ×10⁶ meters, N is the number of initial replibot release sites, and v_repl is the maximum nanoreplicator linear dispersal velocity. For isolated replibots lacking significant aeromotive capabilities, dispersal velocity will be limited approximately to the mean global wind speed, perhaps v_repl ~ 10 m/sec, ignoring the narrow 30-75 m/sec jet streams at 9-16 km altitude [94]. This is also near the maximum feasible velocity for nanorobotic flyers operating in the viscous regime, based on maximum attainable endogenous power densities [6].

Assuming a single initial release site (N = 1) and taking v_repl ~ 10 m/sec, then t_spread ~ 2 ×10⁶ sec. However, a more efficient biosphere conversion strategy would incorporate the simultaneous release of numerous "seed" replibots distributed uniformly throughout the terrestrial biomass, thus reducing the required maximum extension of each expanding replication domain from neighboring replibot release sites. Large numbers of replibots could be transported by high-velocity airborne macroscale carrier vehicles to distant sites around the world and then released, crudely analogous to a jet aircraft scattering printed leaflets over a civilian area during wartime. Nanoreplicator progeny tasked with the conversion of biomass to nanomass within such smaller substrate domains have much less distance to travel to complete their purpose. Minimum biomass conversion time scales roughly as N^½, where N is the number of independent initial replicator domains, as reflected in Table 2 generated from Eqn. 2:
 

This analysis suggests that the limitations on biosphere conversion rate imposed by dispersal velocity are readily overcome by employing a sufficient number of release sites, and do not, by themselves, prohibit ecophagic conversion times on the order of ~1000 seconds or less. In principle, very sophisticated biovores could facultatively aggregate into macroscale assemblages to escape the viscous flight regime, theoretically permitting aerodynamic or even suborbital flight velocities up to 100-1000 m/sec. Replicators incapable of aerial transport will experience significantly longer dispersal times.

In practical surface deployments, major distribution nonuniformities will exist because some areas have significantly larger carbon inventories than others [28]. For example, a map of the global annual net primary production (NPP) of photosynthetically fixed carbon on land shows NPP ranging from 0.1-1.5 kg/m²-yr of carbon, with 25% of the land surface area without permanent ice supporting an NPP > 0.5 kg/m²-yr and an average of 0.426 kg/m²-yr on land [21]. The oceans, which cover ~70% of Earth's surface and show carbon fixation activity averaging only 0.14 kg/m²-yr [21], contain a mere 0.02% of the entire planetary biomass, compared to 99.98% on land.

The uneven geographical distribution of carbon inventories [28] and solar power availability [83] along with possible element shortages (Tables 3 and 5) may produce significant geographical variation in replication rates.  A detailed analysis of such variation is beyond the scope of this paper but likely would place upper limits on replication speed in many environments.
 

5.0 Energy and Materials Requirements Limitations

Interestingly, diamond has the highest known oxidative chemical storage density because it has the highest atom number (and bond) density per unit volume. Organic materials store less energy per unit volume, from ~3 times less than diamond for cholesterol, to ~5 times less for vegetable protein, to ~10-12 times less for amino acids and wood [6]. Since replibots must build energy-rich product structures (e.g. diamondoid) by consuming relatively energy-poor feedstock structures (e.g., biomass), it may not be possible for biosphere conversion to proceed entirely to completion (e.g., all carbon atoms incorporated into nanorobots) using chemical energy alone, even taking into account the possible energy value of the decarbonified sludge byproduct, though such unused carbon may enter the atmosphere as CO₂ and will still be lost to the biosphere.

The speed of biospheric conversion can also be limited by the abundances of chemical elements available in the environment for conversion into nanomass, as compared to the relative quantities of each element that are required by the nanorobot for replication. (In replicator engineering, this is the "materials closure" issue [19];  in chemistry, it is called "stoichiometry.") In the gray goo scenario, nanorobot replication occurs on the Earth's surface, so any elements which are in short supply in the biosphere might alternatively be obtained from nearby topsoil or crustal rocks, although this may impose an additional logistical overhead on replicative processes. Hence only the concentration of the most abundant of these two sources may act as a significant limit to replication speed. Traditional diamondoid nanomachinery designs [4] have employed 8 primary chemical elements, as summarized in Table 3 (more details in Table 5); Table 3 also gives the associated biological [6] and crustal [5] abundances for each element.
 

Dividing the lowest and highest nanorobot requirement by the highest available environmental abundance gives t_mult, the required increase in replication time due to scarcity of a chemical element required for replication. Inspection of Table 3 reveals that sulfur appears to be in the shortest supply relative to nanorobot requirements (at least for current primitive designs), possibly increasing replication time t by a factor of up to 41 while the device waits for sufficient sulfur atoms to be accumulated from the environment. Other elements possibly in somewhat limited supply include P, N and F, although the impact of any of these elements on replication time can probably be minimized by judicious nanorobot composition design choices.

As a general rule, ecophagic nanorobot replication time is longer in direct proportion to the extent that nanorobot elemental requirements exceed the availability of the scarcest element in the consumable substrate, in comparison to the theoretical nanorobot replication time on a perfectly compositionally-matched substrate [19]. This phenomenon is commonplace in biology. For instance, it is well-known that phytoplanktonic growth in the open oceans is iron-limited [29].

The highest near-term risk could come from relatively simple single-behavior replibots whose niche is a high-energy substrate of uniform composition which affords a rapid vector for the dispersal of the replicators [79].  The classic example is tire rubber and asphalt tar binder;  cars, trucks and airplanes roll on roads and tarmacs worldwide. If the ~4 million miles of paved roads in the U.S. [80] represent ~25% of the global total, then road asphalt mass worldwide is ~3 ×10¹³ kg, or ~0.6% M_bio;  the global rubber tire population of ~10 billion tires stockpiled, in use or discarded in scrap heaps [80, 82] adds another ~0.003% M_bio.  Other vectors with similar properties include cotton, polyester or other uniform textiles [79], insulation on electrical wiring, and paper money.  Regular monitoring and decontamination procedures may thwart these threats.
 

6.0 Ecophagic Thermal Pollution Limits (ETPL)

In the crude analysis that follows, we assume that after some number of prior replication cycles, the replibots have converted roughly half of the biosphere to nanoreplicator mass. In the next and final replication cycle, the energy extractable from the remaining half of the global biomass will be consumed as each existing nanorobot replicates itself once more for the last time, thus promptly doubling the existing population and completing the global conversion of biomass into nanomass.

In this case, the total heat energy released at Earth's surface is P_total = P_nano + P_solar, where P_nano is the waste heat generated by the replibots as they emit E_half joules in the last replicative cycle of duration t_last, with P_nano = E_half / t_last, and where the total solar insolation on Earth's cloudless surface that is subsequently thermalized is P_solar ~ 1.75 ×10¹⁷ W. Neglecting the heat-trapping effects of greenhouse gases and the minor contributions from the geological heat flow at Earth's surface, the temperature at the terrestrial surface is given approximately by the Stefan-Boltzmann relation:
 

What is an appropriate maximum operating temperature limit for nanoreplicators that must forage for organic substrate in order to replicate? The softening point for sapphire, an oft-mentioned substitute building material for diamond because of its high strength, high-temperature tolerance, and inability to burn in oxygen, is 2070°K [30], probably near the upper limit in any reasonable ecophagic nanorobot design scenario especially given the seriously negative impact of higher temperatures on nanorobot reliability and functionality (Section 3.0). The combustion temperature of diamond in air is usually given as 870-1070°K [31].

However, such elevated surface temperatures, while perhaps acceptable for diamondoid nanomachines in some circumstances, will immediately volatize and incinerate most of the natural organic feedstock upon which the nanoreplicators must feed. The minimum ignition point of wood, paper, or diesel fuel in air has been given as low as ~500°K [68], and glucose caramelizes [6] at 433°K -- caramelization is not oxidation but rather is a decomposition reaction that includes polymerizations and covalent bondmaking that could render this substrate material somewhat less accessible to the replibots. A still lower temperature threshold is the boiling point of water at 373°K; above this temperature, living things will boil, thus denying ecophagic nanoreplicators access to solution-based chemical processes at normal atmospheric pressures, which could be an important restriction.

The waste heat energy released globally in the last replicative cycle may be estimated as E_half (q D_bio) M_bio, where D_bio is the energy density of the organic feedstock material and q is the energy conversion ratio for its transformation into nanomass. For example, D_bio = 16 MJ/kg for glucose, 17 MJ/kg for vegetable protein, 18 MJ/kg for animal protein, 19 MJ/kg for wood, and 39 MJ/kg for fats [6]. However, these figures refer to the energy content of the organic feedstock, not to the energy that must be consumed (and the waste heat subsequently thermalized) in order to build a kilogram of nanomass. Drexler [4] estimates that the typical energy dissipation caused by chemical transformations involving carbon-rich materials will be E_diss = (q D_bio) ~ 100 MJ/kg of final product using readily-envisioned irreversible methods in systems where low energy dissipation is not a primary design objective. This figure corresponds roughly to the strongest covalent bond energies (e.g., 1190 zJ/bond for C=C, 1327 zJ/bond for C=O, and 1594 zJ/bond for CºC [4]), and is roughly of the same order as the thermodynamic heat of formation of diamond from CO₂(g), ~33 MJ/kg [5].

Drexler [4] claims that energy dissipation may in theory be as low as E_diss ~ 0.1 MJ/kg "if one assumes the development of a set of mechanochemical processes capable of transforming feedstock molecules into complex product structures using only reliable, nearly reversible steps." 0.1 MJ/kg of diamond corresponds roughly to the minimum thermal noise at room temperature (e.g., kT ~ 4 zJ/atom at 298°K). R. Merkle [32] also conjectures that near-zero energy dissipation is in principle possible in certain special circumstances, a possibility that should be investigated in the present context in a future theoretical study. However, near-term nanochemistries are unlikely to be significantly more efficient than natural enzyme chemistries, which have been evolving for efficiency over eons; the terrestrial biosphere fixes ~1.2 ×10¹⁴ kg/yr of biomass carbon [21] with a ~1.4 ×10¹⁴ watt energy input [6], or E_diss ~ 38 MJ/kg of carbon.

Using Eqn. 4, the minimum last-replication time can be calculated for various plausible values of E_diss, wherein the mean terrestrial temperature will not exceed the chosen value of T_Earth during ecophagy, as given in Table 4:
 

^* Actual last-cycle replication time limited to exponential t ~ 100 sec (Section 3.0).

Setting aside Merkle's conjecture, Table 4 suggests that if phenomenally efficient reversible molecular manufacturing techniques become available -- e.g., E_diss ~ 0.1 MJ/kg -- the final replicative cycle of global ecophagy could proceed as quickly as ~1000 seconds while just avoiding incinerating the organic feedstock or boiling environmental water. However, there currently exist no known designs which would be capable of achieving such highly energy-efficient nanoassembly operations.

More probably, highly dissipative molecular manufacturing designs are likely to be implemented during the early and intermediate years of molecular nanotechnology development. Such designs are also likely to be necessary for the very complex machines needed to implement biovorous replication given the enormous variety of chemically diverse natural biological substrates. Assuming current and foreseeable energy-dissipative designs requiring ~100 MJ/kg for chemical transformations (most likely for biovorous systems), complete ecophagy that proceeds slowly enough to add ~4°C to global warming (near the current threshold for immediate climatological detection) will require ~20 months to run to completion. Faster ecophagic devices will run hotter, allowing quicker detection by policing authorities.

The conversion of biomass to nanomass may proceed according to Eqn. 1 up to the ecophagic thermal pollution limit (ETPL) whereupon the specified maximum global temperature T_Earth is attained, after which the replication time must approximately double after each population doubling, ultimately reaching t_last in the final doubling, as described by Eqn. 4. Total time spent in the ETPL-limited regime is ~ 2 t_last. For example, taking t = 100 sec, T_Earth = 300°K, and E_diss ~ 100 MJ/kg, the transition to the ETPL regime occurs when total global nanomass reaches ~5 ×10¹⁰ kg, or only 0.001% of total global biomass, and the last ~17 population doublings remain to be completed over a time span of ~2 t_last = 2 ×10⁷ sec (~7 months). This is also the optimum strategy for an ecophagic population that is attempting to evade premature detection by maintaining a low thermal emissions profile. Constant ecological surveillance for any evidence of ecophagic activity is an appropriate policing measure to provide adequate early warning to the existence of this threat.

Note further that the presence of natural and anthropogenic greenhouse gases in the Earth's atmosphere will amplify any heating effects, helping to make ecophagic activities more immediately visible in its earlier stages. (In theory, a large enough replibot population could actively manage terrestrial albedo or global greenhouse gas concentrations, but these activities would themselves generate still more waste heat.) Additionally, using the actual current mean value of e_r = 0.69 for terrestrial emissivity in Eqns. 3 and 4, rather than the much higher value of e_r = 0.97 for carbon black assumed in calculating Table 4, the last-cycle time t_last increases by another ~40%, giving still more time for defensive instrumentalities to be brought to bear on the situation.

Assuming the surface biomass is compositionally similar to wood (D_bio ~ 19 MJ/kg), prompt consumption (e.g., combustion) of the entire biosphere would release Q_waste = M_bioD_bio ~ 10²³ J of energy.  The combined heat capacity of planetary oceans (1.36 ×10²¹ kg [6] at 4200 J/kg-K [6] = 6 ×10²⁴ J/K) and land (~4 ×10²⁰ kg/km crustal landmass at, e.g., 833 J/kg-K for silica [90] = 3 ×10²³ J/km-K) is ~10²⁵ J/K, so in principle Q_waste, ideally distributed, could be absorbed with a negligible rise in global temperature, ~0.01ºK -- although even a slight rise in ocean temperature could increase mean worldwide humidity, greatly amplifying global warming because water vapor is the most effective greenhouse gas [91].  However, in the instant scenario, the replibots are assumed to be in intimate contact with the biomass which they are consuming -- not with the vast volumes of sea or land.  Air is an excellent insulator (see below), and the thermal conductivity of wood is ~4 times higher than for air, so replication waste heat energy will be conducted primarily into the nanorobot population and the proximate biomass that is being consumed.  The heat capacity of diamond and organic materials (e.g. wood, rubber, etc.) is ~2 MJ/m³-K [6], or ~800 J/kg-K for the biomass/nanomass aggregate as dry mass or up to 3200 J/kg-K assuming 70% water content.  Taking the higher figure, the total heat capacity of the biomass/nanomass aggregate is C_bn ~ 3200 J/kg-K × M_bio = 2 ×10¹⁹ J/K.  Adding Q_waste to this aggregate would raise its temperature by DT ~ Q_waste / C_bn ~ 5,000ºK.

Similarly, air conduction is unlikely to significantly reduce the ETPL limits.  Waste energy can be absorbed by atmospheric heat capacity (5.27 ×10¹⁸ kg [6] at 988 J/kg-K [6] = 5 ×10²¹ J/K) only if said heat energy is delivered to the atmosphere and thoroughly mixed via conduction, convection, or radiative transfer.  But the thermal conductivity (K_t) of air is very poor.  Consider a layer of air H meters thick and area A, with temperature differential DT on opposite faces, with power flow through the layer of P = K_t ADT / H = Q_waste / t_burn, where power is generated by consuming the ecosphere in a time t_burn, so the temperature differential across the layer is DT = Q_waste H / (K_t At_burn). With a thin layer of replibots coating the foliage on Earth's surface, generating heat only on the "skin" of the biosphere, there will be a nonconvective stagnant layer of air trapped for long periods near the surface that is poorly mixed by winds.  In weather modeling this viscous sublayer, called the roughness parameter, may be ~0.01-300 cm thick [84-86] (e.g., 0.01 cm over water surface, 0.1 cm over short grain, 10 cm over prairie grass, 100 cm over grain crops [85]), and sometimes is taken as ~10% of the height of the obstacle [87].  Assuming H ~ 1 cm layer and taking Q_waste = 10²³ J, K_t = 2.5 ×10^-2 W/m-K for air [6], A = 5.10 ×10¹⁴ m² for Earth, then DT ~ 7 ×10⁷ / t_burn.  Thus, consuming M_bio in t_burn = 10⁴ sec nominally produces a temperature differential across the 1 cm layer of DT ~ 7000ºK (and violates our nonconvection assumption);  assuming t_burn = 6 months, then DT ~ 4ºK, detectable using current orbital surveillance assets given a normal tropospheric thermal gradient of 0.006-0.009ºK/m [88].  (Vertical temperature gradients below 0.010ºK/m are considered subadiabatic, producing downward buoyancy forces [89].)  These crude estimates provide an approximate indication of the magnitudes involved, but the details of convective vertical mixing and its possible meteorological consequences during ecophagy are beyond the scope of this paper and should be investigated further.
 

7.0 Homeostatic Resistance to Ecophagy

What is the minimum ecophagic biomass removal rate necessary to overcome the resulting carbon-sequestration response of the natural ecology? One study [20] found that deforestation in the low latitudes during 1990 resulted in forest area expansion and growth in mid- and high-latitude forest that sequestered ~7 ×10¹¹ kg of carbon (e.g., creating ~3 ×10¹² kg of extra biomass) in one year. Estimates of unrealized global forest carbon conservation and sequestration potential suggest a biologic capability of 1-3 ×10¹² kg/yr (e.g., 4-13 ×10¹² kg/yr of biomass) for more than a century [20]. Global oceans are believed to absorb ~2 ×10¹² kg/yr of anthropogenically-produced carbon, creating ~9 ×10¹² kg of new biomass per year [20]. This gives a worldwide carbon sink of ~5 ×10¹² kg/yr of carbon [42] and thus a global biomass recovery of at least ~2 ×10¹³ kg/yr. The upper limit is probably closer to the global net primary biomass production of ~5 ×10¹⁴ kg/yr [21]. (Indeed, natural variations of ~10¹⁴ kg of atmospheric carbon (equivalent to ~6 ×10¹⁴ kg of biomass) were recorded over a ~600-year period during the last three glaciation cycles [43].) Thus it appears that a long-term ecophagic biomass removal rate exceeding 0.2-5 ×10¹⁴ kg/yr (~0.4%-10%/yr of the global biomass) may be necessary to overpower the natural ecological restorative forces.
 

8.0 Additional Scenarios

8.1 Gray Plankton

The gray plankton replicator waste heat signature is readily detected at an early stage. The temperature of most of the ocean is near ~4°C -- for example, ~1.6°C at 3627 m on the floor of Monterey Bay [44]. Typical ocean column thermal gradients are ~0.02°K/m in the top 300 m (1-30 atm) and ~0.006°K/m from 300-1000 m depth (30-100 atm) [44]. A near-seafloor water temperature change of DT = 1°K over a depth range of L = 100 m would be clearly distinguishable from natural variations even using contemporary instrumentation [44], and would evidence an increased seabed power release of I_repl ~ K_t (DT / L) ~ 0.005 W/m², taking thermal conductivity as K_t ~ 0.5 W/m-K for seawater at 4°C. Thus the threshold for seafloor replibot detectability, assuming global seabed area is A_seabed ~ (70%) 4p R_Earth² = 3.6 ×10¹⁴ m², is P_min = I_repl A_seabed ~ 2 ×10¹² watts worldwide or a global replibot population of mass M_min ~ P_mint / E_diss ~ 20 ×10⁶ kg assuming E_diss ~ 100 MJ/kg and t = 1000 sec. (Faster replicators are detectable at lower population masses.) Thus bottom-dwelling gray plankton can be detected before they have consumed more than 10^-9 of the total oceanic abiotic carbon supply.

Direct census sampling of the seafloor may also allow early detection, although nanorobotic samplers will have to contend with a significant number of false targets in the oceanic environment. These false targets may include 0.1 micron small colloids (~7 ×10¹⁴ m^-3) and viruses (~3 ×10¹³ m^-3), 0.2-0.3 micron heterotrophic bacteria (~10¹² m^-3), 0.3 micron large colloids (~10¹³ m^-3), 1 micron cyanobacteria (~10¹⁰ m^-3), 2-3 micron small phytoplankton (~10⁸ m^-3), larger phytoplankton (e.g., 10 micron cells ~10⁶ m^-3), and zooplankton (e.g., 50 micron cells ~10³ m^-3) [36-38]. At the minimum detectable global mass of M_min = 20 ×10⁶ kg estimated above, the number density of gray plankton on the seabed floor is N_gp ~ M_min / (A_seabedm_gp) ~ 2 ×10⁷ m^-2, assuming ~1 micron gray plankton replicators each of mass m_gp ~ 3 ×10^-15 kg. In this scenario, the bottommost 1 mm of the ocean column above the seabed would contain roughly equal numbers of > ~1-micron natural cells and ~1-micron artificial bottom-dwelling gray plankton devices. If not largely confined to the sea floor during most of their replication cycle, the natural cell/device ratio could increase by many orders of magnitude, requiring a more diligent census effort. Census-taking nanorobots can alternatively be used to identify, disable, knapsack or destroy the gray plankton devices.
 

8.2 Gray Dust (Aerovores)

Two independent constraints on gray dust replication speed are materials and energy availability, and both methods suggest that t ~ 10,000 sec for 1-micron replicators and ~1000 sec for 0.1-micron replicators. The analyses are as follows.

First, the mass current M_curr through the surface of a spherical nanorobot of radius R_nanois equal to the number of gas molecules/sec that collide with the fraction f ~ 10% of the nanorobot surface that consists of binding sites for those molecules, times the mass per gas molecule m_gas, divided by the number of collisions required for binding to occur, or N_encounter ~ 100 [6]; that is:
 

where k = 1.381 ×10^-23 J/molecule-K (Boltzmann's constant) and T ~ 300°K is ambient temperature in kelvins. The concentration of gas is c_gas = a_atmT_STPN_A / (V_molarT) (molecules/m³), where a_atm = atmospheric fractional abundance, T_STP = 273.15°K, N_A = 6.023 ×10²³ molecules/mole (Avogadro's number), and molar volume at STP is V_molar = 22.4141 ×10^-3 m³/mole of an ideal gas. The replication time t = M_nano / M_current, where M_nano = (4/3)pra_elementR_nano³, taking r ~ 2000 kg/m³ as nanorobot density and a_element as the fraction of nanorobot mass comprised of a given element. Hence:
 

Taking R_nano = 1 micron and allocating each a_element for the hypothetical CHON replicator as indicated in the second column of Table 6 gives the values of t shown at far right in Table 6. The limiting elements are H and C, but C has the strongest impact on replication time, requiring a t ~ 12,300 sec. Since t scales as R_nano, reducing R_nano to 100 nm reduces t to ~1230 sec for this device. (Mechanical precompression and sortation [6] of gas molecules might reduce t by up to an order of magnitude but may impose partially offsetting internal volume utilization inefficiencies.).
 

Second, the solar energy flux into the nanorobot, assuming that a fraction f of its surface is photosensitive with energy conversion efficiency e, is P_nano = eI_solarf pR_nano². The energy required to build a nanorobot is E_nano ~ M_nano E_diss, hence the replication time is t = E_nano / P_nano, or:
 

Taking r = 2000 kg/m³, E_diss ~ 100 MJ/kg, f = 50%, e = 10%, and I_solar = 100-400 W/m², then for R_nano = 1 micron, t ~ 11,000-53,000 sec; for R_nano = 100 nm, t = 1100-5300 sec.

Since replication of an airborne CHON replibot is primarily carbon-limited, in theory the entire global atmospheric carbon mass of ~5.2 ×10¹⁴ kg C is available for conversion into M_gd = 8.4 ×10¹⁴ kg of CHON nanomass, assuming a 62% carbon content by weight (Table 6). However, because the machines are solar powered, the active population of gray dust nanorobots is restricted to one optical depth of such devices. To a very crude first approximation (e.g., ignoring contributions from scattered and reflected photons), one optical depth occurs when the cumulative cross-sectional area of the nanorobot population equals the surface area of Earth, so the maximum total mass of continuously active CHON airborne nanorobots is:
 

Once the expanding nanorobot population reaches one optical depth (requiring ~0.2% of all atmospheric carbon, or ~3 months of current anthropogenic airborne carbon releases), the replication rate of the gray dust ceases to grow exponentially and becomes essentially constant -- a phenomenon which may be called the "opacity brake effect." (One optical depth of uniformly distributed R_nano = 275 nm aerovores represents a particle number density of ~5 ×10⁸ m^-3.) After the opacity brake point has been reached, a constant nanomass production rate of M_total/t ~ 1.4 ×10⁸ kg/sec ensues until exhaustion of the limiting atmospheric carbon resource. Current instrumentation can detect ~1% variations in the solar constant, so the limit for early bolometric detection is probably ~1% M_total, when ~0.002% of atmospheric carbon has been converted to nanomass.

Dust monitors in late 20th-century wafer-fab clean rooms regularly measure dust densities of ~10 particles/m³ at 0.5 microns and larger [49], potentially allowing detection as early as <10^-8 M_total if more highly discriminating monitors can be developed. If the replibots settle out on the planetary surface and continue replicating there (Section 8.3), they could deprive the ecology of needed sunlight without darkening the sky, but their effects (e.g., a fine gray dust covering everything on the surface) would also be detectable far sooner than the 1% M_total point.

Since replication rate and opacity per unit nanomass vary inversely with R_nano, the most efficient gray dust replibot tasked with opacifying the atmosphere as quickly as possible will have the minimum possible size. (Replication time varies with thickness for a sheetlike nanorobot configuration.) The minimum replibot size is driven by UV radiation damage rates on nanomachinery [4]. Consider the smallest possible replicator with mass M_init = 1.7 ×10^-18 kg (Section 3.0); constructed as a spherical shape of density r = 2000 kg/m³, the radius of this core replicator is R_core ~ 59 nm. The core is surrounded by a radiation shield of thickness d and density ~r. The most dangerous is UV-B at l ~ 280 nm which will conservatively be taken as ~5 W/m² intensity near ground level [4], equivalent to D₀ = 7 ×10¹⁸ photons/m²-sec. The number of bonds cleaved inside the nanorobot is N_cleave = pR_nano²q_yt_lifeD₀exp(-4pk_xd / l), where q_y is quantum yield (bonds cleaved / photons absorbed), t_life is mean time to failure, and k_x is extinction coefficient. k_x ~ 2.26 for graphite at 280 nm [50], a 2250 kg/m³ semimetal that is probably the most UV-absorptive CHON shield material. q_y = 10^-4 to 10^-1 for CHON polymers [51] and various proteins, viruses and phages [52]; following Drexler [4], we adopt q_y ~ 0.01 here (the exact choice is not critical to our conclusions). From Eqn. 7, t ~ c_tR_nano, where c_t ~ 10¹⁰ sec/m. Taking t_life = t n_t, where n_t is the number of offspring constructed before replibot failure, and assuming that N_cleave³ 1 implies device failure [4], then:
 

for replibots that produce n_t offspring before failing. The number of generations needed to replicate one optical depth of nanorobots worldwide, starting from a single device, is n_t = ln(M_total / M_init) = 65-60 for R_nano = 0.1-1 microns. Taking n_t ~ 64, Eqn. 9 defines the smallest gray dust replibot as R_nano ~275 nm (mass ~ 1.7 ×10^-16 kg) with a d ~ 215 nm thick graphite UV shield assuming N_cleave = 1. The smallest replibot that can replicate only once before it fails (e.g., n_t = 1) has R_nano ~ 230 nm with a d ~ 170 nm shield taking N_cleave= 1, or R_nano ~ 175 nm with a d ~ 115 nm shield taking N_cleave = 100 for more robust devices.

From Eqns. 1 and 8, and neglecting dispersal velocity limitations (Section 4.0) the minimum possible time to reach some fraction f_opac of global atmospheric opacity is:
 

For airborne CHON replibots with R_nano = 275 nm and t ~ 2750 sec, 1% of opacity is reached in t_opac ~ 1.85 days, 100% opacity in 2.0 days, leaving a response time of ~3.5 hours between first detection at 1% opacity and complete opacity at 100%. If uniformly distributed throughout the atmosphere, the dust density at 100% opacity would amount to ~0.085 mg/m³ for 275-nm nanorobots, about equal to the typical ~0.05 mg/m³ dust density normally found in the air of most industrialized Western cities [69].

After 100% opacity is reached, another t_end = t (M_gd-M_total) / M_total = 72 days would be required to convert the remaining atmospheric carbon resource into nanomass. However, post-opacity the gray dust replication rate is no longer exponentiating so the defensive nanorobots can quickly catch up.

The most efficient cleanup strategy appears to be the use of air-dropped non-self-replicating nanorobots equipped with prehensile microdragnets. Consider a planetwide dragnet comprised of a square mesh of fibers, with mesh aperture size l_mesh, mesh fibers of thickness d_fiber, and total dragnet area A_net covering Earth's entire surface area A_Earth = 4pR_Earth² = A_net. Minimum fiber thickness is d_fiber (p_airl_mesh² / 4 s_fiber)^½, where p_air 2 atm is the maximum air pressure resisting movement of the net through the air and fiber failure strength is very conservatively taken as s_fiber ~ 10¹⁰ N/m² for carbon nanotubes. For l_mesh 460 nm (smallest possible gray dust replibot, see above), d_fiber 1 nm. Simple geometry gives the total volume of required square-grid dragnet as:
 

Taking A_net = A_Earth = 5.10 ×10¹⁴ m², l_mesh = 460 nm and d_fiber = 1 nm, then V_dragnet = 2200 m³. This dragnet may be carried aloft by a fleet of N_bot = V_dragnet / f_vV_bot spherical defensive nanorobots, each of which uses some fraction f_v of its internal storage volume to hold a piece of the dragnet, where individual defensive nanorobot volume is V_bot = (4/3)p R_nano³. Taking f_v = 5% and R_nano = 0.62 micron, V_bot = 1 micron³ and N_bot = 4.4 ×10²² defensive nanorobots of total mass ~V_botrN_bot = 8.8 ×10⁷ kg (of which ~4.4 ×10⁶ kg is dragnet). Thus a single 88-kg payload of non-self-replicating defensive nanorobots launched from each of 10⁶ deployment sites worldwide (mean site separation ~23 km, ~one per town) to an altitude just above the gray dust replibots can deploy an Earth-covering net, which then descends through the air, selectively filtering out the gray dust replibots. The time required for this dragnet to sweep the entire atmospheric volume of Earth once is t_sweep ~ V_air / (4pR_Earth²v_nano) ~ 24 hours, taking nanorobot aeromotive velocity v_nano ~ 0.1 m/sec for power densities appropriate to solar powered nanodevices [6] and V_air ~ 4.36 ×10¹⁸ m³ at ~1 atm pressure and room temperature. Possible false targets that may be encountered during the sweep include airborne fungal spores at 10-500 m^-3 indoors and 100-1000 m^-3 outdoors [53]; bacteria at 0-500 m^-3 indoors, 179-1083 m^-3 outdoors [53], and ~140 m^-3 up to ~3 km altitude [38]; and inert dust particles of various sizes peaking in number density near ~20 nm [46], in concentrations ranging from 10 m^-3 in semiconductor fab plant clean rooms up to 2 ×10⁷ m^-3 in quiet country air, 6 ×10⁷ m^-3 over residential city air, 1.5 ×10⁸ m^-3 in the worst congested downtown city air, and >2.7 ×10⁸ m^-3 in rooms with smokers present [49, 54]. Of course, multiple cleansing sweeps may be required, insect^a and bird^b management and biocompatibility protocols must be devised, exterior surfaces must be appropriately hydrophobic to avoid providing condensation nuclei for cloud and fog formation, and so forth.

The total machine volume of one optical depth of 275-nm gray dust replibots is 1.9 ×10⁸ m³, making an average cleanup requirement of only ~4500 micron³ of targets per defensive nanorobot. A spherical knapsack comprised of additional mesh material having an enclosed volume of 4500 micron³ adds only 11% to the onboard mesh storage requirement. Each defensive nanorobot deploys a (110 micron)² ~ 12,100 micron² section of the planetwide dragnet. In theory, if this section were curved into a huge spherical knapsack, it would make a storage volume of 125,000 micron³ -- enough to hold the equivalent of ~28 optical depths of gray dust replibots during passage through locally dense clouds of target airborne nanoreplicators.

Each defensive nanorobot requires ~66 nN of motive force and ~6600 pW of onboard power to overcome drag loss [6] on the ~5.3 cm length of dragnet fiber that it is passing through the air at 0.1 m/sec. This power is provided by a rear-deployed, 30% efficient, 55 micron², ~10 nm thick solar collector film that stows in a 0.55 micron³ volume before deployment and adds only ~45 pW to drag power after deployment. When fully deployed, the defensive fleet contributes <0.5% additional atmospheric opacity, and clears air for an energy cost of ~5.8 J/m³ of contaminated atmosphere, per pass. Defensive nanorobot locomotion in the viscous flight regime may be provided by screw drives, viscous anchoring via the prehensile dragnet, or other means [6].

The Stokes settling velocity [6] in air is ~240 micron/sec for R_nano = 1 micron, ~20 micron/sec for R_nano = 275 nm and ~5 micron/sec for R_nano = 100 nm, giving 10-km passive fall times (in still atmosphere) of 1.3 years, 16 years and 67 years, respectively.

Alternative airborne or ground-based atmospheric filtration configurations that could permit more rapid filtering are readily envisioned. For example, since drag power varies as the square of the velocity, then by increasing mesh volume 10,000-fold while decreasing airflow velocity 100-fold, total drag power remains unchanged but whole-atmosphere turnover proceeds 100-fold faster, e.g., ~15 minutes.

^b  There are ~300 billion birds on Earth [70].  The average bird devotes 20% of body volume to its respiratory system [71], mostly unidirectional airflow unlikely to permanently trap gray dust, but ~20% of the bird respiratory volume consists of tidally exchanged air in 8-9 anterior and posterior air sacs, and air spaces in the bones [71]. Assuming dead air in birds represents 38% of tidal volume as in humans [6], and that the average bird is ~500 cm³ in volume, then the worldwide bird population has ~2 × 10⁶ m³ of respiratory dead air which could accumulate ~10¹³ aerovores if the birds are  breathing replibot-contaminated air at a concentration equivalent to ~1% atmospheric opacity.  In this case ~10^-12 of the global aerovore population resides  inside bird respiratory systems (~40 replibots per bird at 1% opacity), necessitating specialized quarantine or inspection and release protocols for birds passing through the dragnet.  Under similar exposure, ~7 × 10¹³ aerovores (~3 × 10^-12 of the total) could reside in all human lungs worldwide, or ~10,000 aerovores/person.
 

8.3 Gray Lichens

The growth rate of gray lichens on the surface of the Earth will be primarily energy-limited, not materials-limited. While it is true that chemolithotrophic microorganisms such as Thiobacillus ferrooxidans use reduced iron and sulfur compounds for their energy source [60-62], and that other chemolithotrophic bacteria can metabolize inorganic carbon (e.g., assimilating CO₂ from carbonate rock) using a pathway similar to green plants [63], such energy sources appear to be far less plentiful than ambient sunlight because most rocks are already fully oxidized. (For example, the oxidation of Fe⁺⁺ to Fe⁺⁺⁺ by chemolithotrophs liberates only 0.75 MJ/kg [64], as compared to ~16 MJ/kg for the combustion of glucose in oxygen [6].) Assuming that up to 400 W/m² in the visible spectrum is harvested with 30% efficiency for 8 hours/day over the entire landmass of Earth, the 6 ×10¹⁵ watts theoretically available could produce at most ~6 ×10⁷ kg/sec of mineral nanomass taking E_diss ~ 100 MJ/kg as before. Even assuming an optimal dispersal pattern, ~2.6 years would be required for the growing mineral nanomass to equal the terrestrial b